This record contains data collected on the charter vessel Rum Rambler between 15 and 19 August 2004 in the Fitzroy River Estuary. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments and retrieved chlorophyll a estimate, the absorption coefficient for dissolved (aCDOM) particulate (a/p) and detrital or non-algal (a/d) components of the water column. 20 stations were sampled. The data was used primarily to validate ocean colour sensors MERIS, MODIS and SeaWIFs and the SST sensor AATSR. Samples were collected and analysed for pigments, total suspended solids (TSS) and dissolved and particulate absorption coefficients. CLW deployed instruments RAMSES, ac-9, Hydroscat and Hydrorad.